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Effects of SHP2 on <t>cardiomyocyte</t> pyroptosis. (A) Representative western blots and quantitative analysis of the relative protein expression levels of IL-1β and GSDMD in BMMs. GAPDH served as the loading control. (B) Flow cytometry analysis of cell apoptosis rates in HL-1 cells. Data are expressed as the mean ± standard deviation. *P<0.05 and **P<0.01. ns, not significant; SHP2, Src homology region 2-containing protein tyrosine phosphatase 2; GSDMD, gasdermin D; BMMs, bone marrow-derived macrophages; WT, wild type; KO, knockout; BRD4, bromodomain-containing protein 4; NC, negative control; shRNA, short hairpin RNA.
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Effects of SHP2 on <t>cardiomyocyte</t> pyroptosis. (A) Representative western blots and quantitative analysis of the relative protein expression levels of IL-1β and GSDMD in BMMs. GAPDH served as the loading control. (B) Flow cytometry analysis of cell apoptosis rates in HL-1 cells. Data are expressed as the mean ± standard deviation. *P<0.05 and **P<0.01. ns, not significant; SHP2, Src homology region 2-containing protein tyrosine phosphatase 2; GSDMD, gasdermin D; BMMs, bone marrow-derived macrophages; WT, wild type; KO, knockout; BRD4, bromodomain-containing protein 4; NC, negative control; shRNA, short hairpin RNA.
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Effects of SHP2 on cardiomyocyte pyroptosis. (A) Representative western blots and quantitative analysis of the relative protein expression levels of IL-1β and GSDMD in BMMs. GAPDH served as the loading control. (B) Flow cytometry analysis of cell apoptosis rates in HL-1 cells. Data are expressed as the mean ± standard deviation. *P<0.05 and **P<0.01. ns, not significant; SHP2, Src homology region 2-containing protein tyrosine phosphatase 2; GSDMD, gasdermin D; BMMs, bone marrow-derived macrophages; WT, wild type; KO, knockout; BRD4, bromodomain-containing protein 4; NC, negative control; shRNA, short hairpin RNA.

Journal: Molecular Medicine Reports

Article Title: Myeloid SHP2 attenuates myocardial ischemia‑reperfusion injury via regulation of BRD4/SYK/STING/NOX4/NLRP3 signaling

doi: 10.3892/mmr.2025.13520

Figure Lengend Snippet: Effects of SHP2 on cardiomyocyte pyroptosis. (A) Representative western blots and quantitative analysis of the relative protein expression levels of IL-1β and GSDMD in BMMs. GAPDH served as the loading control. (B) Flow cytometry analysis of cell apoptosis rates in HL-1 cells. Data are expressed as the mean ± standard deviation. *P<0.05 and **P<0.01. ns, not significant; SHP2, Src homology region 2-containing protein tyrosine phosphatase 2; GSDMD, gasdermin D; BMMs, bone marrow-derived macrophages; WT, wild type; KO, knockout; BRD4, bromodomain-containing protein 4; NC, negative control; shRNA, short hairpin RNA.

Article Snippet: The HL-1 mouse cardiomyocyte cell line was purchased from Wuhan Procell Life Technology Co., Ltd.

Techniques: Western Blot, Expressing, Control, Flow Cytometry, Standard Deviation, Derivative Assay, Knock-Out, Negative Control, shRNA